Journal: JCI Insight

Article Title: Increased expression of cathepsin C in airway epithelia exacerbates airway remodeling in asthma

doi: 10.1172/jci.insight.181219

Figure Lengend Snippet: ( A ) The mRNA expression of CTSC in the induced sputum of patients with asthma and HCs. Data are presented as mean ± SEM. One-way ANOVA followed by Tukey’s post hoc test was used. ( B and C ) Pearson’s correlation analysis between CTSC expression and pulmonary function in patients with asthma. FEV 1 , forced expiratory volume in 1 second; FVC, forced vital capacity. ( D ) Pearson’s correlation analysis between CTSC expression and ACT score in patients with asthma. ( E – H ) Pearson’s correlation analysis between CTSC expression and related parameters of airway remodeling in patients with asthma. ( I ) Receiver operating characteristic analysis for the expression of CTSC to discriminate HCs from patients with SA. ( J ) Receiver operating characteristic analysis for the expression of CTSC to discriminate patients with MMA from patients with SA. * P < 0.05; *** P < 0.001.

Article Snippet: Recombinant human CTSC (rhCTSC, 1071-CY-010, R&D Systems) was activated according to the manufacturer’s instruction ( ).

Techniques: Expressing

Journal: JCI Insight

Article Title: Increased expression of cathepsin C in airway epithelia exacerbates airway remodeling in asthma

doi: 10.1172/jci.insight.181219

Figure Lengend Snippet: ( A ) Cell proliferation analysis of HBECs after CSTC silencing and overexpression using CCK-8 assay ( n = 5). Two-way ANOVA followed by Tukey’s post hoc test was used. ( B ) Proliferation curves of HBECs after CSTC silencing and overexpression ( n = 4). Two-way ANOVA followed by Tukey’s post hoc test was used. ( C ) A scratch test for evaluating damage repair capability in HBECs ( n = 3). Two-way ANOVA followed by Tukey’s post hoc test was used. ( D ) Effects of CTSC expression on the permeability of HBECs monolayers ( n = 3). One-way ANOVA followed by Tukey’s post hoc test was used. ( E ) Effects of CTSC expression on ROS generation ( n = 4–8). One-way ANOVA followed by Bonferroni’s post hoc test was used. ( F and G ) Representative immunofluorescence of HBECs stained for E-cad and ZO-1 after CSTC overexpression ( n = 3; scale bar: 50 μm). All data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Recombinant human CTSC (rhCTSC, 1071-CY-010, R&D Systems) was activated according to the manufacturer’s instruction ( ).

Techniques: Over Expression, CCK-8 Assay, Expressing, Permeability, Immunofluorescence, Staining

Journal: JCI Insight

Article Title: Increased expression of cathepsin C in airway epithelia exacerbates airway remodeling in asthma

doi: 10.1172/jci.insight.181219

Figure Lengend Snippet: ( A ) The secretion of CTSC in HBECs was detected by ELISA ( n = 4). One-way ANOVA followed by Tukey’s post hoc test was used. ( B ) Proliferation curves of HLF-1 after rhCTSC stimulation ( n = 4). Two-way ANOVA followed by Tukey’s post hoc test was used. ( C ) Expression of α-SMA and COL 1 protein was detected by Western blot ( n = 3). Two-way ANOVA followed by Tukey’s post hoc test was used. ( D ) Expression of p38, p-p38, Erk, p-Erk, JNK, and p-JNK protein was detected by Western blot ( n = 3). Unpaired t test was used. ( E ) HLF-1 were pretreated with p38 inhibitor SB203580 and then stimulated with rhCTSC. Expression of α-SMA and COL 1 protein was detected by Western blot ( n = 3). Two-way ANOVA followed by Tukey’s post hoc test was used. All data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Recombinant human CTSC (rhCTSC, 1071-CY-010, R&D Systems) was activated according to the manufacturer’s instruction ( ).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot